data/YM_0852/README.md

Pembrolizumab-scFv Optimiziation Variants Iter0 x PD-1 (YM_0852)

Overview

YM_852 are Pembrolizumab mutations against its native target; we introduce mutations that include deletions, insertions, double and single mutations to quantify the sensitivity of the local landscape in engaging the native target.

Experimental details

This dataset includes 29883 unique scFvs and 1 unique native target sequence. A more extensive methods section can be found in our publication here.

Misc dataset details

We define the following binders:

A-library (scFvs)

There are several terms you can filter by:

• WT_<i>: These are WT replicates.
• del_<i>: A deletion from the WT
• ins_<i>: An insertion from WT
• pair_<i>: A double mutation (2 residues mutated from WT)
• single_<i>: A single mutation

To get the mutations of interest relative to the parent, we recommend an alignment to the WT sequence.

Alpha-library

There is only 1 sequence, which is the native target.